› 3igd

X-ray diffraction
2.4Å resolution

Crystal structure of Mtu recA intein, splicing domain

Released:
DOI: 10.2210/pdb3igd/pdb
PDB entry 3igd coloured by chain, front view.
PDB entry 3igd coloured by chain, side view.
PDB entry 3igd coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center.
Hiraga K, Soga I, Dansereau JT, Pereira B, Derbyshire V, Du Z, Wang C, Van Roey P, Belfort G, Belfort M
J Mol Biol 393 1106-17 (2009)
PMID: 19744499

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-MtuI Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 15.44 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHJ3 (Residues: 252-691; Coverage: 18%)
Gene names: MTV002.02c, Rv2737c, recA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand SNN 1 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 90.15Å b: 90.15Å c: 39.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.289 0.289 0.337
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Interactions between Hedgehog proteins and their binding partners come into view.
Beachy et al. (2010)
5 more

1 mention without citation

Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center.
Hiraga et al. (2009)