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X-ray diffraction
1.83Å resolution

Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a

Released:
DOI: 10.2210/pdb3ign/pdb
PDB entry 3ign coloured by chain, front view.
PDB entry 3ign coloured by chain, side view.
PDB entry 3ign coloured by chain, top view.
Source organism: Marinobacter nauticus VT8
Primary publication:
Crystal structure of a catalytically active GG(D/E)EF diguanylate cyclase domain from Marinobacter aquaeolei with bound c-di-GMP product.
Vorobiev SM, Neely H, Yu B, Seetharaman J, Xiao R, Acton TB, Montelione GT, Hunt JF
J Struct Funct Genomics 13 177-83 (2012)
PMID: 22843345

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.46 KDa
Source organism: Marinobacter nauticus VT8
Expression system: Escherichia coli
UniProt:
  • Canonical: A1U3W3 (Residues: 149-316; Coverage: 53%)
Gene name: Maqu_2607
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand C2E 2 x C2E
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P43212
Unit cell:
a: 59.229Å b: 59.229Å c: 118.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial diguanylate cyclases: structure, function and mechanism in exopolysaccharide biofilm development.
Whiteley et al. (2015)
4 other articles

13 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
12 more