› 3igr

X-ray diffraction
2Å resolution

The Crystal Structure of Ribosomal-protein-S5-alanine Acetyltransferase from Vibrio fischeri to 2.0A

Released:
DOI: 10.2210/pdb3igr/pdb
PDB entry 3igr coloured by chain, front view.
PDB entry 3igr coloured by chain, side view.
PDB entry 3igr coloured by chain, top view.
Source organism: Aliivibrio fischeri ES114
Entry authors: Stein AJ, Sather A, Shackelford G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an N-terminal L-alanyl-[S5 protein of 30S ribosome] = CoA + an N-terminal N-acetyl-L-alanyl-[S5 protein of 30S ribosome]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[ribosomal protein uS5]-alanine N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.84 KDa
Source organism: Aliivibrio fischeri ES114
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5DZH6 (Residues: 1-184; Coverage: 100%)
Gene names: VF_A0750, rimJ
Sequence domains: Acetyltransferase (GNAT) domain
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 63.504Å b: 107.173Å c: 145.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.237
Expression system: Escherichia coli BL21(DE3)

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