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X-ray diffraction
1.5Å resolution

Structure of the Salmonella enterica N-acetylmannosamine-6-phosphate 2-epimerase

Released:
DOI: 10.2210/pdb3igs/pdb
PDB entry 3igs coloured by chain, front view.
PDB entry 3igs coloured by chain, side view.
PDB entry 3igs coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Entry authors: Anderson SM, Wawrzak Z, Gordon E, Skarina T, Papazisi L, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N-acetylmannosamine-6-phosphate 2-epimerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 24.59 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZLQ7 (Residues: 1-229; Coverage: 100%)
Gene names: STM3337, nanE2
Sequence domains: Putative N-acetylmannosamine-6-phosphate epimerase
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand 16G 1 x 16G
Ligand SO4 14 x SO4
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 183.29Å b: 63.32Å c: 41.8Å
α: 90° β: 91.28° γ: 90°
R-values:
R R work R free
0.149 0.147 0.177
Expression system: Escherichia coli

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