› 3ihi

X-ray diffraction
1.94Å resolution

Crystal structure of mouse thymidylate synthase

Released:
DOI: 10.2210/pdb3ihi/pdb
PDB entry 3ihi coloured by chain, front view.
PDB entry 3ihi coloured by chain, side view.
PDB entry 3ihi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mouse thymidylate synthase does not show the inactive conformation, observed for the human enzyme
Dowiercial A, Jarmula A, Wilk P, Rypniewski W, Kowalska M, Fraczyk T, Ciesla J, Rode W
Struct Chem (2016)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07607 (Residues: 1-307; Coverage: 100%)
Gene name: Tyms
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2
Unit cell:
a: 158.202Å b: 87.962Å c: 68.043Å
α: 90° β: 97.43° γ: 90°
R-values:
R R work R free
0.221 0.22 0.266
Expression system: Escherichia coli

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