› 3ihl

X-ray diffraction
2.8Å resolution

Human CTPS2 crystal structure

Released:
DOI: 10.2210/pdb3ihl/pdb
PDB entry 3ihl coloured by chain, front view.
PDB entry 3ihl coloured by chain, side view.
PDB entry 3ihl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Moche M, Siponen MI, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Karlberg T, Kotenyova T, Kotzsch A, Kragh Nielsen T, Nyman T, Persson C, Roos AK, Sagemark J, Schueler H, Schutz P, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Welin M, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRF8 (Residues: 1-275; Coverage: 47%)
Gene name: CTPS2
Sequence domains: CTP synthase N-terminus
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6222
Unit cell:
a: 168.25Å b: 168.25Å c: 132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.232
Expression system: Escherichia coli

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