› 3ii3

X-ray diffraction
2.7Å resolution

Structure of ORF157 from Acidianus filamentous Virus 1

Released:
DOI: 10.2210/pdb3ii3/pdb
PDB entry 3ii3 coloured by chain, front view.
PDB entry 3ii3 coloured by chain, side view.
PDB entry 3ii3 coloured by chain, top view.
Source organism: Acidianus filamentous virus 1
Primary publication:
ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease.
Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichière J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C
J Virol 84 5025-31 (2010)
PMID: 20200253

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative uncharacterized protein Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.86 KDa
Source organism: Acidianus filamentous virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70LE6 (Residues: 2-157; Coverage: 99%)
Gene name: ORF157
Sequence domains: Protein of unknown function DUF3258
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P3121
Unit cell:
a: 65.445Å b: 65.445Å c: 85.604Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.271 0.27 0.281
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The influence of external factors on bacteriophages--review.
Jończyk et al. (2011)
6 more

2 mentions without citation

Computational design of a protein-based enzyme inhibitor.
Procko et al. (2013)
1 more