› 3iio

X-ray diffraction
2.25Å resolution

Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series

Released:
DOI: 10.2210/pdb3iio/pdb
PDB entry 3iio coloured by chain, front view.
PDB entry 3iio coloured by chain, side view.
PDB entry 3iio coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series.
Khersonsky O, Röthlisberger D, Dym O, Albeck S, Jackson CJ, Baker D, Tawfik DS
J Mol Biol 396 1025-42 (2010)
PMID: 20036254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KE07 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 262 amino acids
Theoretical weight: 29.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P31
Unit cell:
a: 106.584Å b: 106.584Å c: 128.753Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.278
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Beyond directed evolution--semi-rational protein engineering and design.
Lutz S. (2010)
40 more

2 mentions without citation

Optimizing non-natural protein function with directed evolution.
Brustad et al. (2011)
1 more