› 3ij3

X-ray diffraction
1.8Å resolution

1.8 Angstrom Resolution Crystal Structure of Cytosol Aminopeptidase from Coxiella burnetii

Released:
DOI: 10.2210/pdb3ij3/pdb
PDB entry 3ij3 coloured by chain, front view.
PDB entry 3ij3 coloured by chain, side view.
PDB entry 3ij3 coloured by chain, top view.
Source organism: Coxiella burnetii
Entry authors: Minasov G, Halavaty A, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosol aminopeptidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 53.69 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83DX0 (Residues: 1-458; Coverage: 100%)
Gene name: CBU_0572
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand CL 1 x CL
Ligand LI 1 x LI
Ligand SO4 5 x SO4
Ligand EDO 3 x EDO
Ligand PEG 1 x PEG
Ligand PGE 2 x PGE
Ligand PG4 3 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P321
Unit cell:
a: 112.803Å b: 112.803Å c: 78.089Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.177
Expression system: Escherichia coli

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