› 3ijd

X-ray diffraction
2Å resolution

Uncharacterized protein Cthe_2304 from Clostridium thermocellum binds two copies of 5-methyl-5,6,7,8-tetrahydrofolic acid

Released:
DOI: 10.2210/pdb3ijd/pdb
PDB entry 3ijd coloured by chain, front view.
PDB entry 3ijd coloured by chain, side view.
PDB entry 3ijd coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Entry authors: Bonanno JB, Gilmore M, Bain KT, Miller S, Ozyurt S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.31 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DHS7 (Residues: 2-305; Coverage: 99%)
Gene name: Cthe_2304
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

1 bound ligand:
Ligand C2F 4 x C2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 51.581Å b: 52.368Å c: 60.918Å
α: 94.68° β: 97.68° γ: 90.57°
R-values:
R R work R free
0.221 0.218 0.265
Expression system: Escherichia coli BL21(DE3)

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