› 3ijm

X-ray diffraction
1.7Å resolution

The structure of a restriction endonuclease-like fold superfamily protein from Spirosoma linguale.

Released:
DOI: 10.2210/pdb3ijm/pdb
PDB entry 3ijm coloured by chain, front view.
PDB entry 3ijm coloured by chain, side view.
PDB entry 3ijm coloured by chain, top view.
Source organism: Spirosoma linguale DSM 74
Entry authors: Cuff ME, Tesar C, Samano S, Bearden J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Restriction endonuclease domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17 KDa
Source organism: Spirosoma linguale DSM 74
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D2QNN3 (Residues: 1-148; Coverage: 100%)
Gene name: Slin_4590
Structure domains: tt1808, chain A

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 65.77Å b: 65.849Å c: 68.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.199
Expression system: Escherichia coli BL21

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