› 3ikt

X-ray diffraction
2.26Å resolution

Crystal structure of a Rex-family repressor/DNA/NAD+ complex from Thermus aquaticus

Released:
DOI: 10.2210/pdb3ikt/pdb
PDB entry 3ikt coloured by chain, front view.
PDB entry 3ikt coloured by chain, side view.
PDB entry 3ikt coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural basis for NADH/NAD+ redox sensing by a Rex family repressor.
McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, Paget MS, Kielkopf CL
Mol Cell 38 563-75 (2010)
PMID: 20513431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116412 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Redox-sensing transcriptional repressor Rex Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.73 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72I39 (Residues: 1-206; Coverage: 98%)
Gene names: TT_C1293, rex
Sequence domains:
Structure domains:
Rex operator DNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NAD 1 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P3121
Unit cell:
a: 63.15Å b: 63.15Å c: 299.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Genetically encoded fluorescent redox sensors.
Lukyanov et al. (2014)
10 more

8 mentions without citation

How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.
Lee et al. (2021)
7 more