› 3il2

X-ray diffraction
2.49Å resolution

Crystal structure of a Rex-family repressor R90D mutant/DNA complex from Thermus aquaticus

Released:
DOI: 10.2210/pdb3il2/pdb
PDB entry 3il2 coloured by chain, front view.
PDB entry 3il2 coloured by chain, side view.
PDB entry 3il2 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural basis for NADH/NAD+ redox sensing by a Rex family repressor.
McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, Paget MS, Kielkopf CL
Mol Cell 38 563-75 (2010)
PMID: 20513431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116413 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Redox-sensing transcriptional repressor Rex Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.78 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72I39 (Residues: 1-206; Coverage: 98%)
Gene names: TT_C1293, rex
Sequence domains:
Structure domains:
Rex operator DNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 7.01 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 4 x MSE
Ligand BRU 8 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P3121
Unit cell:
a: 62.95Å b: 62.95Å c: 298.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.254 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Genetically encoded fluorescent redox sensors.
Lukyanov et al. (2014)
11 more

1 mention without citation

How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.
Lee et al. (2021)