› 3ilk

X-ray diffraction
2.01Å resolution

The structure of a probable methylase family protein from Haemophilus influenzae Rd KW20

Released:
DOI: 10.2210/pdb3ilk/pdb
PDB entry 3ilk coloured by chain, front view.
PDB entry 3ilk coloured by chain, side view.
PDB entry 3ilk coloured by chain, top view.
Source organism: Haemophilus influenzae
Entry authors: Tan K, Li H, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized tRNA/rRNA methyltransferase HI_0380 Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 27.88 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44676 (Residues: 1-241; Coverage: 100%)
Gene name: HI_0380
Sequence domains: SpoU rRNA Methylase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand ACT 1 x ACT
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 48.167Å b: 94.392Å c: 124.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli

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