› 3imf

X-ray diffraction
1.99Å resolution

1.99 Angstrom resolution crystal structure of a short chain dehydrogenase from Bacillus anthracis str. 'Ames Ancestor'

Released:
DOI: 10.2210/pdb3imf/pdb
PDB entry 3imf coloured by chain, front view.
PDB entry 3imf coloured by chain, side view.
PDB entry 3imf coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Halavaty AS, Minasov G, Skarina T, Onopriyenko O, Gordon E, Peterson S, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase, short chain dehydrogenase/reductase family Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 28.42 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8NTZ6 (Residues: 1-254; Coverage: 100%)
Gene name: GBAA_4204
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 174.237Å b: 89.56Å c: 71.785Å
α: 90° β: 111.6° γ: 90°
R-values:
R R work R free
0.168 0.166 0.206
Expression system: Escherichia coli

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