› 3imh

X-ray diffraction
1.76Å resolution

CRYSTAL STRUCTURE OF GALACTOSE 1-EPIMERASE FROM Lactobacillus acidophilus NCFM

Released:
DOI: 10.2210/pdb3imh/pdb
PDB entry 3imh coloured by chain, front view.
PDB entry 3imh coloured by chain, side view.
PDB entry 3imh coloured by chain, top view.
Source organism: Lactobacillus acidophilus NCFM
Entry authors: Patskovsky Y, Toro R, Dickey M, Chang S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose-1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 38.07 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FJ46 (Residues: 2-328; Coverage: 100%)
Gene names: LBA1457, galM
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand CL 3 x CL
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4212
Unit cell:
a: 134.683Å b: 134.683Å c: 103.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.17
Expression system: Escherichia coli

Quick links