› 3in2

X-ray diffraction
2.6Å resolution

Crystal structure of the N47S/M121L variant of Pseudomonas aeruginosa azurin in the Cu(II) state

Released:
DOI: 10.2210/pdb3in2/pdb
PDB entry 3in2 coloured by chain, front view.
PDB entry 3in2 coloured by chain, side view.
PDB entry 3in2 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Rationally tuning the reduction potential of a single cupredoxin beyond the natural range.
Marshall NM, Garner DK, Wilson TD, Gao YG, Robinson H, Nilges MJ, Lu Y
Nature 462 113-6 (2009)
PMID: 19890331

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.92 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 49.66Å b: 49.66Å c: 105.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.266
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Copper active sites in biology.
Solomon et al. (2014)
22 more

1 mention without citation

Protein design: toward functional metalloenzymes.
Yu et al. (2014)