› 3ios

X-ray diffraction
1.6Å resolution

Structure of MTB dsbF in its mixed oxidized and reduced forms

Released:
DOI: 10.2210/pdb3ios/pdb
PDB entry 3ios coloured by chain, front view.
PDB entry 3ios coloured by chain, side view.
PDB entry 3ios coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
An extracellular disulfide bond forming protein (DsbF) from Mycobacterium tuberculosis: structural, biochemical, and gene expression analysis.
Chim N, Riley R, The J, Im S, Segelke B, Lekin T, Yu M, Hung LW, Terwilliger T, Whitelegge JP, Goulding CW
J Mol Biol 396 1211-26 (2010)
PMID: 20060836

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.43 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53924 (Residues: 47-182; Coverage: 75%)
Gene name: MT1716
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P42212
Unit cell:
a: 100.12Å b: 100.12Å c: 30.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.144 0.2
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Redox homeostasis in mycobacteria: the key to tuberculosis control?
Kumar et al. (2011)
4 more

5 mentions without citation

The TB Structural Genomics Consortium: a decade of progress.
Chim et al. (2011)
4 more