› 3ipi

X-ray diffraction
1.9Å resolution

Crystal Structure of a Geranyltranstransferase from the Methanosarcina mazei

Released:
DOI: 10.2210/pdb3ipi/pdb
PDB entry 3ipi coloured by chain, front view.
PDB entry 3ipi coloured by chain, side view.
PDB entry 3ipi coloured by chain, top view.
Source organism: Methanosarcina mazei
Entry authors: Kumaran D, Mohammed MB, Brown A, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranylfarnesyl diphosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.3 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PYS1 (Residues: 12-295; Coverage: 96%)
Gene name: MM_0789
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand MLA 6 x MLA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 68.16Å b: 81.273Å c: 49.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.251
Expression system: Escherichia coli BL21(DE3)

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