› 3iqt

X-ray diffraction
1.4Å resolution

Structure of the HPT domain of Sensor protein barA from Escherichia coli CFT073.

Released:
DOI: 10.2210/pdb3iqt/pdb
PDB entry 3iqt coloured by chain, front view.
PDB entry 3iqt coloured by chain, side view.
PDB entry 3iqt coloured by chain, top view.
Source organism: Escherichia coli CFT073
Entry authors: Cuff ME, Rakowski E, Kim Y, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal transduction histidine-protein kinase BarA Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.94 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AEC6 (Residues: 799-918; Coverage: 13%)
Gene names: barA, c3349
Sequence domains: Hpt domain
Structure domains: HPT domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand BTB 1 x BTB
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 24.316Å b: 60.359Å c: 37.156Å
α: 90° β: 108.21° γ: 90°
R-values:
R R work R free
0.143 0.141 0.181
Expression system: Escherichia coli BL21

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