› 3iqw

X-ray diffraction
3Å resolution

AMPPNP complex of C. therm. Get3

Released:
DOI: 10.2210/pdb3iqw/pdb
PDB entry 3iqw coloured by chain, front view.
PDB entry 3iqw coloured by chain, side view.
PDB entry 3iqw coloured by chain, top view.
Source organism: Chaetomium thermophilum
Primary publication:
Structural insights into tail-anchored protein binding and membrane insertion by Get3.
Bozkurt G, Stjepanovic G, Vilardi F, Amlacher S, Wild K, Bange G, Favaloro V, Rippe K, Hurt E, Dobberstein B, Sinning I
Proc Natl Acad Sci U S A 106 21131-6 (2009)
PMID: 19948960

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anion-transporting ATPase-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.13 KDa
Source organism: Chaetomium thermophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SFE0 (Residues: 13-339; Coverage: 97%)
Gene name: CTHT_0061710
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 46.57Å b: 105.73Å c: 136.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.271
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
18 more

7 mentions without citation

The complex process of GETting tail-anchored membrane proteins to the ER.
Chartron et al. (2012)
6 more