› 3ir6

X-ray diffraction
2.8Å resolution

Crystal structure of NarGHI mutant NarG-H49S

Released:
DOI: 10.2210/pdb3ir6/pdb
PDB entry 3ir6 coloured by chain, front view.
PDB entry 3ir6 coloured by chain, side view.
PDB entry 3ir6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Protein crystallography reveals a role for the FS0 cluster of Escherichia coli nitrate reductase A (NarGHI) in enzyme maturation.
Rothery RA, Bertero MG, Spreter T, Bouromand N, Strynadka NC, Weiner JH
J Biol Chem 285 8801-7 (2010)
PMID: 20053990

Function and Biology Details

Reaction catalysed: 
Nitrate + a quinol = nitrite + a quinone + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-140570 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Respiratory nitrate reductase 1 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 1247 amino acids
Theoretical weight: 140.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P09152 (Residues: 1-1247; Coverage: 100%)
Gene names: JW1215, b1224, bisD, narC, narG
Sequence domains:
Structure domains:
Respiratory nitrate reductase 1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 512 amino acids
Theoretical weight: 58.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P11349 (Residues: 1-512; Coverage: 100%)
Gene names: JW1216, b1225, narH
Sequence domains:
Structure domains:
Respiratory nitrate reductase 1 gamma chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 25.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P11350 (Residues: 1-225; Coverage: 100%)
Gene names: JW1218, b1227, chlI, narI
Sequence domains: Nitrate reductase gamma subunit
Structure domains: Transmembrane di-heme cytochromes, Chain C

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
4 bound ligands:
Ligand GDP 2 x GDP
Ligand AGA 1 x AGA
Ligand SF4 3 x SF4
Ligand F3S 1 x F3S
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 154.142Å b: 239.825Å c: 139.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.249
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Emerging critical roles of Fe-S clusters in DNA replication and repair.
Fuss et al. (2015)
7 more

3 mentions without citation

Structural and mechanistic insights on nitrate reductases.
Coelho et al. (2015)
2 more