› 3ish

X-ray diffraction
2.43Å resolution

Crystal structure of Helicobacter pylori thioredoxin reductase

Released:
DOI: 10.2210/pdb3ish/pdb
PDB entry 3ish coloured by chain, front view.
PDB entry 3ish coloured by chain, side view.
PDB entry 3ish coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Entry authors: Sanders D, Obiero J, van Straaten K

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 33.58 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P56431 (Residues: 1-311; Coverage: 100%)
Gene names: HP_0825, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 3 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3221
Unit cell:
a: 89.4Å b: 89.4Å c: 279.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.252
Expression system: Escherichia coli

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