› 3iso

X-ray diffraction
1.9Å resolution

Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry

Released:
DOI: 10.2210/pdb3iso/pdb
PDB entry 3iso coloured by chain, front view.
PDB entry 3iso coloured by chain, side view.
PDB entry 3iso coloured by chain, top view.
Source organism: Clonorchis sinensis
Primary publication:
A histidine substitution confers metal binding affinity to a Schistosoma japonicum Glutathione S-transferase.
Han YH, Seo HA, Kim GH, Lee CK, Kang YK, Ryu KH, Chung YJ
Protein Expr Purif 73 74-7 (2010)
PMID: 20347989

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.07 KDa
Source organism: Clonorchis sinensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25595 (Residues: 1-218; Coverage: 100%)
Gene name: CSKR_111935
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P3221
Unit cell:
a: 96.39Å b: 96.39Å c: 115.428Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.226
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Antioxidant enzymes activities of Burkholderia spp. strains-oxidative responses to Ni toxicity.
Dourado et al. (2015)
2 more