› 3ist

X-ray diffraction
1.65Å resolution

Crystal structure of glutamate racemase from Listeria monocytogenes in complex with succinic acid

Released:
DOI: 10.2210/pdb3ist/pdb
PDB entry 3ist coloured by chain, front view.
PDB entry 3ist coloured by chain, side view.
PDB entry 3ist coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Entry authors: Majorek KA, Chruszcz M, Skarina T, Onopriyenko O, Stam J, Anderson WF, Savchenko A, Bujnicki JM, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
L-glutamate = D-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.8 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y7N7 (Residues: 1-266; Coverage: 100%)
Gene names: lmo1237, murI, racE
Sequence domains: Asp/Glu/Hydantoin racemase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SIN 2 x SIN
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 66.42Å b: 66.42Å c: 258.774Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.211
Expression system: Escherichia coli BL21(DE3)

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