› 3itd

X-ray diffraction
1.89Å resolution

Crystal structure of an inactive 17beta-Hydroxysteroid dehydrogenase (Y167F mutated form) from fungus Cochliobolus lunatus

Released:
DOI: 10.2210/pdb3itd/pdb
PDB entry 3itd coloured by chain, front view.
PDB entry 3itd coloured by chain, side view.
PDB entry 3itd coloured by chain, top view.
Source organism: Curvularia lunata
Entry authors: Cassetta A, Lamba D, Krastanova I

Function and Biology Details

Reaction catalysed: 
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-131691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17beta-hydroxysteroid dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.92 KDa
Source organism: Curvularia lunata
Expression system: Escherichia coli
UniProt:
  • Canonical: O93874 (Residues: 1-270; Coverage: 100%)
Gene name: 17HSDcl
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4122
Unit cell:
a: 67.79Å b: 67.79Å c: 269.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.189
Expression system: Escherichia coli

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