› 3iti

X-ray diffraction
1.55Å resolution

Structure of bovine trypsin with the MAD triangle B3C

Released:
DOI: 10.2210/pdb3iti/pdb
PDB entry 3iti coloured by chain, front view.
PDB entry 3iti coloured by chain, side view.
PDB entry 3iti coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
How to get the magic triangle and the MAD triangle into your protein crystal.
Beck T, da Cunha CE, Sheldrick GM
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1068-70 (2009)
PMID: 19851024

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand BRV 1 x BRV
Ligand BEN 1 x BEN
Ligand CA 1 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 53.658Å b: 56.883Å c: 66.808Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 not available 0.213

Quick links

Citations

1 review citation

An Overview of Microcrystal Electron Diffraction (MicroED).
Mu et al. (2021)
3 other articles

3 mentions without citation

The structure of human ubiquitin in 2-methyl-2,4-pentanediol: a new conformational switch.
Huang et al. (2011)
2 more