› 3itj

X-ray diffraction
2.4Å resolution

Crystal structure of Saccharomyces cerevisiae thioredoxin reductase 1 (Trr1)

Released:
DOI: 10.2210/pdb3itj/pdb
PDB entry 3itj coloured by chain, front view.
PDB entry 3itj coloured by chain, side view.
PDB entry 3itj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae W303
Primary publication:
Insights into the specificity of thioredoxin reductase-thioredoxin interactions. A structural and functional investigation of the yeast thioredoxin system.
Oliveira MA, Discola KF, Alves SV, Medrano FJ, Guimarães BG, Netto LE
Biochemistry 49 3317-26 (2010)
PMID: 20235561

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 338 amino acids
Theoretical weight: 36.32 KDa
Source organism: Saccharomyces cerevisiae W303
Expression system: Escherichia coli
UniProt:
  • Canonical: P29509 (Residues: 2-319; Coverage: 100%)
Gene names: D9476.5, TRR1, YDR353W
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
Ligand CIT 6 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: C2
Unit cell:
a: 127.975Å b: 135.411Å c: 75.819Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.171 0.169 0.194
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Roles of Peroxiredoxin and Thioredoxin in Hydrogen Peroxide Sensing and in Signal Transduction.
Netto et al. (2016)
4 more

6 mentions without citation

Comparative genomics allowed the identification of drug targets against human fungal pathogens.
Abadio et al. (2011)
5 more