› 3ius

X-ray diffraction
1.66Å resolution

The structure of a functionally unknown conserved protein from Silicibacter pomeroyi DSS

Released:
DOI: 10.2210/pdb3ius/pdb
PDB entry 3ius coloured by chain, front view.
PDB entry 3ius coloured by chain, side view.
PDB entry 3ius coloured by chain, top view.
Source organism: Ruegeria pomeroyi DSS-3
Entry authors: Tan K, Tesar C, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.29 KDa
Source organism: Ruegeria pomeroyi DSS-3
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LM08 (Residues: 1-283; Coverage: 100%)
Gene name: SPO3755
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand FMT 2 x FMT
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 57.736Å b: 81.648Å c: 58.655Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.219 0.217 0.257
Expression system: Escherichia coli

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