› 3iv8

X-ray diffraction
2.53Å resolution

N-acetylglucosamine-6-phosphate deacetylase from Vibrio cholerae complexed with fructose 6-phosphate

Released:
DOI: 10.2210/pdb3iv8/pdb
PDB entry 3iv8 coloured by chain, front view.
PDB entry 3iv8 coloured by chain, side view.
PDB entry 3iv8 coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Osipiuk J, Maltseva N, Stam J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
N-acetyl-D-glucosamine 6-phosphate + H(2)O = D-glucosamine 6-phosphate + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylglucosamine-6-phosphate deacetylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 41.27 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: O32445 (Residues: 1-378; Coverage: 100%)
Gene names: VC_0994, nagA
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand F6P 1 x F6P
Ligand NI 4 x NI
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 83.181Å b: 123.047Å c: 156.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.253
Expression system: Escherichia coli

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