› 3ivk

X-ray diffraction
3.1Å resolution

Crystal Structure of the Catalytic Core of an RNA Polymerase Ribozyme Complexed with an Antigen Binding Antibody Fragment

Released:
DOI: 10.2210/pdb3ivk/pdb
PDB entry 3ivk coloured by chain, front view.
PDB entry 3ivk coloured by chain, side view.
PDB entry 3ivk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the catalytic core of an RNA-polymerase ribozyme.
Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, Bartel DP
Science 326 1271-5 (2009)
PMID: 19965478

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-212923 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Fab light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 213 amino acids
Theoretical weight: 23.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 224 amino acids
Theoretical weight: 23.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
class I ligase product Chains: C, M
Molecule details ›
Chains: C, M
Length: 128 nucleotides
Theoretical weight: 41.3 KDa

Ligands and Environments

3 bound ligands:
Ligand CD 2 x CD
Ligand MG 33 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 207.499Å b: 206.452Å c: 135.926Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.225
Expression system: Escherichia coli

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Citations

19 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
18 more

18 mentions without citation

New molecular engineering approaches for crystallographic studies of large RNAs.
Zhang et al. (2014)
17 more