› 3ixq

X-ray diffraction
1.78Å resolution

Structure of ribose 5-phosphate isomerase a from methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb3ixq/pdb
PDB entry 3ixq coloured by chain, front view.
PDB entry 3ixq coloured by chain, side view.
PDB entry 3ixq coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603).
Strange RW, Antonyuk SV, Ellis MJ, Bessho Y, Kuramitsu S, Yokoyama S, Hasnain SS
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1214-7 (2009)
PMID: 20054114

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 226 amino acids
Theoretical weight: 24.86 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58998 (Residues: 1-226; Coverage: 100%)
Gene names: MJ1603, rpiA
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand PGO 11 x PGO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P21
Unit cell:
a: 54.856Å b: 100.273Å c: 80.465Å
α: 90° β: 92.54° γ: 90°
R-values:
R R work R free
0.15 0.148 0.188
Expression system: Escherichia coli

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
1 other article

2 mentions without citation

The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603).
Strange et al. (2009)
1 more