› 3iyw

Electron Microscopy
13.7Å resolution

West Nile virus in complex with Fab fragments of MAb CR4354 (fitted coordinates of envelope proteins and Fab fragments of one icosahedral ASU)

Released:
DOI: 10.2210/pdb3iyw/pdb
PDB entry 3iyw coloured by chain, front view.
PDB entry 3iyw coloured by chain, side view.
PDB entry 3iyw coloured by chain, top view.
Source organisms:
Primary publication:
Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.
Kaufmann B, Vogt MR, Goudsmit J, Holdaway HA, Aksyuk AA, Chipman PR, Kuhn RJ, Diamond MS, Rossmann MG
Proc Natl Acad Sci U S A 107 18950-5 (2010)
PMID: 20956322
Related structures: EMD-5190

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 420-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242377 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 400 amino acids
Theoretical weight: 43.27 KDa
Source organism: West Nile virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q91R02 (Residues: 1-400; Coverage: 94%)
Sequence domains:
CR4354 Fab fragment X1, heavy chain H Chains: H, K
Molecule details ›
Chains: H, K
Length: 230 amino acids
Theoretical weight: 24.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CR4354 Fab fragment X1, light chain L Chains: L, M
Molecule details ›
Chains: L, M
Length: 220 amino acids
Theoretical weight: 22.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, FUL
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
Resolution: 13.7Å
Relevant EMDB volumes: EMD-5190
Expression system: Homo sapiens

Quick links

Citations

32 review citations

The human antibody response to dengue virus infection.
Wahala et al. (2011)
31 more

8 mentions without citation

Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.
Kaufmann et al. (2010)
7 more