› 3iz4

Electron Microscopy
13.6Å resolution

Modified E. coli tmRNA in the resume state with the tRNA-like domain in the ribosomal P site interacting with the SmpB

Released:
DOI: 10.2210/pdb3iz4/pdb
PDB entry 3iz4 coloured by chain, front view.
PDB entry 3iz4 coloured by chain, side view.
PDB entry 3iz4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Visualizing the transfer-messenger RNA as the ribosome resumes translation.
Fu J, Hashem Y, Wower I, Lei J, Liao HY, Zwieb C, Wower J, Frank J
EMBO J 29 3819-25 (2010)
PMID: 20940705
Related structures: EMD-5234

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186031 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
SsrA-binding protein Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 14.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RR57 (Residues: 2-123; Coverage: 85%)
Gene names: TTHA0902, smpB
Sequence domains: SmpB protein
Modified E. coli transfer-messenger RNA Chain: A
Molecule details ›
Chain: A
Length: 377 nucleotides
Theoretical weight: 121.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: transfer-messenger RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.6Å
Relevant EMDB volumes: EMD-5234
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The bacterial translation stress response.
Starosta et al. (2014)
11 more

8 mentions without citation

Visualizing the transfer-messenger RNA as the ribosome resumes translation.
Fu et al. (2010)
7 more