› 3j04

Electron Microscopy
20Å resolution

EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state

Released:
DOI: 10.2210/pdb3j04/pdb
PDB entry 3j04 coloured by chain, front view.
PDB entry 3j04 coloured by chain, side view.
PDB entry 3j04 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Phosphorylated smooth muscle heavy meromyosin shows an open conformation linked to activation.
Baumann BA, Taylor DW, Huang Z, Tama F, Fagnant PM, Trybus KM, Taylor KA
J Mol Biol 415 274-87 (2012)
PMID: 22079364
Related structures: EMD-5257

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135959 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin-11 Chains: A, D
Molecule details ›
Chains: A, D
Length: 909 amino acids
Theoretical weight: 104.66 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-910; Coverage: 46%)
Gene name: MYH11
Sequence domains:
Myosin regulatory light chain 2, smooth muscle major isoform Chains: B, E
Molecule details ›
Chains: B, E
Length: 143 amino acids
Theoretical weight: 16.58 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02612 (Residues: 26-168; Coverage: 83%)
Sequence domains: EF-hand domain pair
Myosin light polypeptide 6 Chains: C, F
Molecule details ›
Chains: C, F
Length: 148 amino acids
Theoretical weight: 16.65 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 4-151; Coverage: 98%)
Gene name: MYL6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Relevant EMDB volumes: EMD-5257
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Structure, interactions and function of the N-terminus of cardiac myosin binding protein C (MyBP-C): who does what, with what, and to whom?
Pfuhl et al. (2012)
4 more

10 mentions without citation

Synthetic biology approaches to dissecting linear motor protein function: towards the design and synthesis of artificial autonomous protein walkers.
Linke et al. (2020)
9 more