› 3j4k

Electron Microscopy
8Å resolution

Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state

Released:
DOI: 10.2210/pdb3j4k/pdb
PDB entry 3j4k coloured by chain, front view.
PDB entry 3j4k coloured by chain, side view.
PDB entry 3j4k coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state.
Sousa DR, Stagg SM, Stroupe ME
J Mol Biol 425 4544-55 (2013)
PMID: 24021812
Related structures: EMD-5751

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159327 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
tropomyosin Chains: F, G
Molecule details ›
Chains: F, G
Length: 136 amino acids
Theoretical weight: 11.59 KDa
Source organism: Gallus gallus

Ligands and Environments

1 bound ligand:
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-5751

Quick links

Citations

5 review citations

Targeting the sarcomere to correct muscle function.
Hwang et al. (2015)
4 more

2 mentions without citation

Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state.
Sousa et al. (2013)
1 more