› 3ja7

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution

Released:
DOI: 10.2210/pdb3ja7/pdb
PDB entry 3ja7 coloured by chain, front view.
PDB entry 3ja7 coloured by chain, side view.
PDB entry 3ja7 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution.
Sun L, Zhang X, Gao S, Rao PA, Padilla-Sanchez V, Chen Z, Sun S, Xiang Y, Subramaniam S, Rao VB, Rossmann MG
Nat Commun 6 7548 (2015)
PMID: 26144253
Related structures: EMD-6324

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Portal protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 455 amino acids
Theoretical weight: 53.36 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13334 (Residues: 74-524; Coverage: 86%)
Gene name: 20
Sequence domains: Bacteriophage T4-like portal protein (Gp20)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-6324
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Mechanisms of DNA Packaging by Large Double-Stranded DNA Viruses.
Rao et al. (2015)
13 more

14 mentions without citation

Current approaches for the fitting and refinement of atomic models into cryo-EM maps using CCP-EM.
Nicholls et al. (2018)
13 more