› 3jaw

Electron Microscopy
3.9Å resolution

Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP)

Released:
DOI: 10.2210/pdb3jaw/pdb
PDB entry 3jaw coloured by chain, front view.
PDB entry 3jaw coloured by chain, side view.
PDB entry 3jaw coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.
Zhang R, Alushin GM, Brown A, Nogales E
Cell 162 849-59 (2015)
PMID: 26234155
Related structures: EMD-6354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135917 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GSP 2 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-6354

Quick links

Citations

46 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
45 more

1 mention without citation

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.
Zhang et al. (2015)