› 3jb7

Electron Microscopy
4Å resolution

In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus

Released:
DOI: 10.2210/pdb3jb7/pdb
PDB entry 3jb7 coloured by chain, front view.
PDB entry 3jb7 coloured by chain, side view.
PDB entry 3jb7 coloured by chain, top view.
Source organism: Bombyx mori cypovirus 1
Primary publication:
In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus.
Zhang X, Ding K, Yu X, Chang W, Sun J, Zhou ZH
Nature 527 531-534 (2015)
PMID: 26503045
Related structures: EMD-6404

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-111975 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
RdRp catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1225 amino acids
Theoretical weight: 138.81 KDa
Source organism: Bombyx mori cypovirus 1
UniProt:
  • Canonical: D0EZK6 (Residues: 1-1225; Coverage: 100%)
Viral structural protein 4 Chain: B
Molecule details ›
Chain: B
Length: 561 amino acids
Theoretical weight: 63.68 KDa
Source organism: Bombyx mori cypovirus 1
UniProt:
  • Canonical: Q9IR43 (Residues: 1-561; Coverage: 100%)
VP1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 amino acids
Theoretical weight: 2.73 KDa
Source organism: Bombyx mori cypovirus 1
UniProt:
  • Canonical: D3JWE6 (Residues: 111-134; Coverage: 2%)
RNA (5'-R(P*GP*GP*GP*GP*GP*G)-3') Chain: t
Molecule details ›
Chain: t
Length: 6 nucleotides
Theoretical weight: 2.03 KDa
RNA (5'-R(P*CP*CP*CP*CP*C)-3') Chain: m
Molecule details ›
Chain: m
Length: 5 nucleotides
Theoretical weight: 1.48 KDa

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand CTP 1 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.001Å
Relevant EMDB volumes: EMD-6404

Quick links

Citations

15 review citations

Unravelling biological macromolecules with cryo-electron microscopy.
Fernandez-Leiro et al. (2016)
14 more

2 mentions without citation

In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus.
Zhang et al. (2015)
1 more