› 3jbj

Electron Microscopy
7.6Å resolution

Cryo-EM reconstruction of F-actin

Released:
DOI: 10.2210/pdb3jbj/pdb
PDB entry 3jbj coloured by chain, front view.
PDB entry 3jbj coloured by chain, side view.
PDB entry 3jbj coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Kim LY, Thompson PM, Lee HT, Pershad M, Campbell SL, Alushin GM
J Mol Biol 428 10-25 (2016)
PMID: 26493222
Related structures: EMD-6448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.6Å
Relevant EMDB volumes: EMD-6448

Quick links

Citations

7 review citations

Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.
Izard et al. (2016)
6 more

1 mention without citation

The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Kim et al. (2016)