› 3jbl

Electron Microscopy
4.5Å resolution

Cryo-EM Structure of the Activated NAIP2/NLRC4 Inflammasome Reveals Nucleated Polymerization

Released:
DOI: 10.2210/pdb3jbl/pdb
PDB entry 3jbl coloured by chain, front view.
PDB entry 3jbl coloured by chain, side view.
PDB entry 3jbl coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization.
Zhang L, Chen S, Ruan J, Wu J, Tong AB, Yin Q, Li Y, David L, Lu A, Wang WL, Marks C, Ouyang Q, Zhang X, Mao Y, Wu H
Science 350 404-9 (2015)
PMID: 26449474
Related structures: EMD-6458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NLR family CARD domain-containing protein 4 Chains: A, B, C, D, E, F, G, H, I, J, K
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K
Length: 932 amino acids
Theoretical weight: 106.24 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3UP24 (Residues: 93-1024; Coverage: 91%)
Gene names: Card12, Ipaf, Nlrc4
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 11 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-6458
Expression system: Spodoptera frugiperda

Quick links

Citations

94 review citations

Inflammasomes: mechanism of assembly, regulation and signalling.
Broz et al. (2016)
93 more

19 mentions without citation

Animal NLRs provide structural insights into plant NLR function.
Bentham et al. (2017)
18 more