› 3jrs

X-ray diffraction
2.05Å resolution

Crystal structure of (+)-ABA-bound PYL1

Released:
DOI: 10.2210/pdb3jrs/pdb
PDB entry 3jrs coloured by chain, front view.
PDB entry 3jrs coloured by chain, side view.
PDB entry 3jrs coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis of abscisic acid signalling.
Miyazono K, Miyakawa T, Sawano Y, Kubota K, Kang HJ, Asano A, Miyauchi Y, Takahashi M, Zhi Y, Fujita Y, Yoshida T, Kodaira KS, Yamaguchi-Shinozaki K, Tanokura M
Nature 462 609-14 (2009)
PMID: 19855379

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abscisic acid receptor PYL1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 208 amino acids
Theoretical weight: 23.97 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VZS8 (Residues: 8-211; Coverage: 92%)
Gene names: At5g46790, MZA15.21, PYL1, RCAR12
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand A8S 3 x A8S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 97.73Å b: 97.73Å c: 135.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.229
Expression system: Escherichia coli

Quick links

Citations

67 review citations

Abscisic acid: emergence of a core signaling network.
Cutler et al. (2010)
66 more

6 mentions without citation

Molecular basis of the core regulatory network in ABA responses: sensing, signaling and transport.
Umezawa et al. (2010)
5 more