› 3jru

X-ray diffraction
2.6Å resolution

Crystal structure of Leucyl Aminopeptidase (pepA) from Xoo0834,Xanthomonas oryzae pv. oryzae KACC10331

Released:
DOI: 10.2210/pdb3jru/pdb
PDB entry 3jru coloured by chain, front view.
PDB entry 3jru coloured by chain, side view.
PDB entry 3jru coloured by chain, top view.
Source organism: Xanthomonas oryzae pv. oryzae
Entry authors: Natarajan S, Huynh K-H, Kang LW

Function and Biology Details

Reactions catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable cytosol aminopeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 51.08 KDa
Source organism: Xanthomonas oryzae pv. oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5H4N2 (Residues: 1-490; Coverage: 100%)
Gene names: XOO0834, pepA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CO3 2 x CO3
Ligand ZN 4 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P213
Unit cell:
a: 152.126Å b: 152.126Å c: 152.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.215
Expression system: Escherichia coli

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