› 3jsb

X-ray diffraction
2.13Å resolution

Crystal structure of the N-terminal domain of the Lymphocytic Choriomeningitis Virus L protein

Released:
DOI: 10.2210/pdb3jsb/pdb
PDB entry 3jsb coloured by chain, front view.
PDB entry 3jsb coloured by chain, side view.
PDB entry 3jsb coloured by chain, top view.
Source organism: Lymphocytic choriomeningitis virus (strain Armstrong)
Primary publication:
The N-terminal domain of the arenavirus L protein is an RNA endonuclease essential in mRNA transcription.
Morin B, Coutard B, Lelke M, Ferron F, Kerber R, Jamal S, Frangeul A, Baronti C, Charrel R, de Lamballerie X, Vonrhein C, Lescar J, Bricogne G, Günther S, Canard B
PLoS Pathog 6 e1001038 (2010)
PMID: 20862324

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.72 KDa
Source organism: Lymphocytic choriomeningitis virus (strain Armstrong)
Expression system: Escherichia coli
UniProt:
  • Canonical: P14240 (Residues: 2-197; Coverage: 9%)
Gene names: L, Segment L
Sequence domains: Arenavirus cap snatching domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 145.01Å b: 159.35Å c: 52.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.222
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Common and unique features of viral RNA-dependent polymerases.
te Velthuis AJ. (2014)
28 more

12 mentions without citation

Conventional and unconventional mechanisms for capping viral mRNA.
Decroly et al. (2011)
11 more