› 3jsj

X-ray diffraction
2.1Å resolution

Crystal structure of a putative tetr-transcriptional regulator (sav143) from streptomyces avermitilis ma-4680 at 2.10 A resolution

Released:
DOI: 10.2210/pdb3jsj/pdb
PDB entry 3jsj coloured by chain, front view.
PDB entry 3jsj coloured by chain, side view.
PDB entry 3jsj coloured by chain, top view.
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 20.67 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82RK0 (Residues: 1-189; Coverage: 100%)
Gene name: SAVERM_143
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 56.401Å b: 84.351Å c: 103.39Å
α: 90° β: 102.31° γ: 90°
R-values:
R R work R free
0.191 0.189 0.23
Expression system: Escherichia coli

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