› 3ju6

X-ray diffraction
2.45Å resolution

Crystal Structure of Dimeric Arginine Kinase in Complex with AMPPNP and Arginine

Released:
DOI: 10.2210/pdb3ju6/pdb
PDB entry 3ju6 coloured by chain, front view.
PDB entry 3ju6 coloured by chain, side view.
PDB entry 3ju6 coloured by chain, top view.
Source organism: Apostichopus japonicus
Primary publication:
Structural basis for a reciprocating mechanism of negative cooperativity in dimeric phosphagen kinase activity.
Wu X, Ye S, Guo S, Yan W, Bartlam M, Rao Z
FASEB J 24 242-52 (2010)
PMID: 19783784

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 42.77 KDa
Source organism: Apostichopus japonicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XY07 (Residues: 1-370; Coverage: 100%)
Gene name: AK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ARG 1 x ARG
Ligand ANP 1 x ANP
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 86.823Å b: 60.073Å c: 139.946Å
α: 90° β: 93.08° γ: 90°
R-values:
R R work R free
0.225 0.214 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Natural Products Containing 'Rare' Organophosphorus Functional Groups.
Petkowski et al. (2019)
18 other articles

3 mentions without citation

The protein common interface database (ProtCID)--a comprehensive database of interactions of homologous proteins in multiple crystal forms.
Xu et al. (2011)
2 more