› 3jwg

X-ray diffraction
1.9Å resolution

Crystal structure analysis of the methyltransferase domain of bacterial-CtHen1-C

Released:
DOI: 10.2210/pdb3jwg/pdb
Model geometry
Fit model/data
PDB entry 3jwg coloured by chain, front view.
PDB entry 3jwg coloured by chain, side view.
PDB entry 3jwg coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1.
Mui Chan C, Zhou C, Brunzelle JS, Huang RH
Proc Natl Acad Sci U S A 106 17699-704 (2009)
PMID: 19822745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small RNA 2'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.47 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DJ37 (Residues: 258-465; Coverage: 45%)
Gene name: Cthe_2767
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 83.995Å b: 83.995Å c: 61.995Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Regulation of small RNA stability: methylation and beyond.
Ji et al. (2012)
5 more

5 mentions without citation

Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1.
Mui Chan et al. (2009)
4 more