› 3jxb

X-ray diffraction
1.67Å resolution

Crystal structure of the P22 c2 repressor protein in complex with synthetic operator 9C

Released:
DOI: 10.2210/pdb3jxb/pdb
PDB entry 3jxb coloured by chain, front view.
PDB entry 3jxb coloured by chain, side view.
PDB entry 3jxb coloured by chain, top view.
Source organism: Lederbergvirus P22
Primary publication:
Sequence recognition of DNA by protein-induced conformational transitions.
Watkins D, Mohan S, Koudelka GB, Williams LD
J Mol Biol 396 1145-64 (2010)
PMID: 20053356

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116454 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein C2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 67 amino acids
Theoretical weight: 7.49 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli
UniProt:
  • Canonical: P69202 (Residues: 2-68; Coverage: 31%)
Gene name: C2
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*CP*AP*TP*TP*TP*AP*AP*GP*AP*CP*GP*TP*CP*TP*TP*AP*AP*AP*TP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided
5'-D(*TP*AP*TP*TP*TP*AP*AP*GP*AP*CP*GP*TP*CP*TP*TP*AP*AP*AP*TP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 40.602Å b: 54.319Å c: 114.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 citation in other articles

Control of DNA minor groove width and Fis protein binding by the purine 2-amino group.
Hancock et al. (2013)
18 more

4 mentions without citation

Noncanonical DNA-binding mode of repressor and its disassembly by antirepressor.
Kim et al. (2016)
3 more