› 3jy6

X-ray diffraction
1.97Å resolution

Crystal structure of LacI Transcriptional regulator from Lactobacillus brevis

Released:
DOI: 10.2210/pdb3jy6/pdb
PDB entry 3jy6 coloured by chain, front view.
PDB entry 3jy6 coloured by chain, side view.
PDB entry 3jy6 coloured by chain, top view.
Source organism: Levilactobacillus brevis ATCC 367
Entry authors: Syed Ibrahim B, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 31.07 KDa
Source organism: Levilactobacillus brevis ATCC 367
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03T70 (Residues: 64-328; Coverage: 81%)
Gene name: LVIS_0444
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 40.57Å b: 109.328Å c: 113.342Å
α: 90° β: 95.17° γ: 90°
R-values:
R R work R free
0.247 0.247 0.261
Expression system: Escherichia coli BL21(DE3)

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