› 3jyq

X-ray diffraction
1.16Å resolution

Quinate dehydrogenase from Corynebacterium glutamicum in complex with shikimate and NADH

Released:
DOI: 10.2210/pdb3jyq/pdb
PDB entry 3jyq coloured by chain, front view.
PDB entry 3jyq coloured by chain, side view.
PDB entry 3jyq coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity, and discrimination.
Höppner A, Schomburg D, Niefind K
Biol Chem 394 1505-16 (2013)
PMID: 23929881

Function and Biology Details

Reaction catalysed: 
Quinate + NAD(+) = 3-dehydroquinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinate/shikimate dehydrogenase (NAD(+)) Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 29.72 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X5C9 (Residues: 1-283; Coverage: 100%)
Gene names: aroE, qsuD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand SKM 1 x SKM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2
Unit cell:
a: 120.92Å b: 63.39Å c: 35.67Å
α: 90° β: 94.34° γ: 90°
R-values:
R R work R free
0.15 0.149 0.174
Expression system: Escherichia coli BL21

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Citations

1 review citation

The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework.
Peek et al. (2015)
4 other articles